AFM / SPM eSeminars
These recorded seminars feature researchers and experts presenting various AFM/SPM principles, technologies, applications, and performance-optimizing tips.
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Imaging in Liquids with AFM
Running time: 63 minutes
March 26, 2009
Speakers: Arvind Raman, PhD Purdue University, School of Mechanical Engineering; W. Travis Johnson, PhD Research Scientist, Agilent Technologies
"VEDA: a free online simulation tool for the AFM community"
VEDA (Virtual environment for dynamic AFM) is a suite of free, cyber-enabled tools available on www.nanohub.org that simulate tapping mode AFM using magnetic/acoustic excitation (MAC/AAC) on organic and inorganic surfaces in liquids as well as ambient conditions. In this seminar, a brief introduction to the tools and their use in choosing cantilevers and operating conditions for optimal imaging will be presented.
"Imaging in Liquids with AFM"
Unlike competing nanoscale imaging techniques, Atomic Force Microscopy (AFM) can be used to obtain high resolution images of samples under a wide variety of environments. This includes air, reactive or inert gases, solvents, aqueous buffers and even cell growth media; the latter two of which are critical to maintain the viability of many biological samples. In this seminar, the advantages and some of the challenges of imaging samples under liquids will be presented
AFM Image Optimization
Running time: 61 minutes
December, 16, 2008
Speakers: Michael Serry, Gilbert Min, Agilent Technologies, Inc.
Collect the best AFM images possible! This seminar will address key topics such as recognizing a reasonable image; a low-fidelity image; or an image that is useless because it has many artifacts. It will identify some common artifacts, and the options that may exist to eliminate them or to reduce their impact on your measurements
We will demonstrate how to filter images for enhanced visualization and ways to improve the accuracy of a measurement. We will also discuss the use of post processing software for image data analysis, filtering for high-quality images, and filtering for metrological data.
Higher Harmonic Imaging with AFM
Running time: 37 minutes
May 28, 2008
Speakers: Peter Hinterdorfer,PhD University of Linz & Gerald Kada Agilent Technologies
Learn all about the utilization of this exciting new imaging mode for surface characterization and life science studies! At this seminar, we will discuss how harmonic imaging works, the range of information it can provide about your sample, and harmonic-related data collection and analysis.
AFM Imaging in Liquids
Running time: 39 minutes
May 7, 2008
Speakers: David Kaftan, PhD University of South Bohemia & Gerald Kada PhD Agilent Technologies
Attaining high resolution AFM images in liquid can be challenging. This seminar will discuss AFM imaging techniques for soft samples in aqueous buffers and how to obtain detailed structural information for biological samples in their native, physiological environments - For example, nucleic acids, proteins, lipids, eukaryotic and prokaryotic cells and subcellular structures.
AFM Image Optimization & Studies of Local Electric Properties
Running time: 63 minutes
April 10, 2008
Speaker: Sergei Magonov PhD, Agilent Technologies
Optimization of Imaging in Atomic Force Microscopy -General aspects of imaging in contact and oscillatory modes will be discussed with emphasis on rational analysis of AFM images. Dependence of AFM images on experimental parameters will be reviewed. Introduction to Studies of Local Electric Properties with Atomic Force Microscopy - AFM measurements of the local electric properties are known for almost 20 years, current interest in these applications is driven by improved electronic detection and development of new modes. Single-pass techniques (Electric Force Microscopy, Kevin Force Microscopy, Scanning Piezoresponse Microscopy, etc.) offering new capabilities for visualization local charges, conducting paths, dopant mapping will be reviewed.
Sample Preparation for AFM
Running time: 57 minutes
March 27, 2008
Speakers: David Allison PhD University of Tennessee, Knoxville & Song Xu PhD Agilent Technologies
Proper sample preparation is a prelude to AFM imaging success! This seminar will cover many important aspects of sample preparation, including how to calculate the correct concentration of a sample, how to prepare living cells for imaging in liquid, and how to affix a sample to a puck for imaging in air. Common misconceptions concerning sample preparation will also be discussed.
Choosing the Correct Cantilever for Your Application
Running time: 70 minutes
October 18, 2007
Speakers: Philip Russell PhD Appalachian State University & Sergei Magonov PhD Agilent Technologies
Choosing the correct cantilever can be very confusing and waste your valuable research time. This seminar will help you understand the types of cantilevers on the market and explain how to choose the best one for your samples and how to optimize data.