Chapter 2
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| Such methods are very computer intensive and it can take a long time to analyze a single image. For example, to do the blind reconstruction on a single 256 X 256 pixel image with an 3 GHz Pentium PC can take 10 minutes. |
| Probe Damage |
The quality of an AFM image is critically dependent on the shape of the probe used for measuring an image. The AFM probe can be severely damaged by tip approach (see Section 3.3). Handling the probe incorrectly before it is placed in the microscope can also cause probe damage. For example, if the probe is exposed to high electric fields, the probe tip can be blown off by electrostatic discharge. Finally, the probe tip can get dirty from the packing materials used to hold the probe while shipping. |
Multiple Probes |
The scan rate of an AFM limits the sizes of areas that can be analyzed to
a few hundred microns at best. It would be highly desirable to create an
AFM with multiple probes that could scan many areas simultaneously.
Several efforts to create “multiple probe” atomic force microscopes
demonstrated that it is possible. In the first approach, several AFM
scanners were positioned above a silicon wafer and scanned independently.
In the second approach, several probes on a silicon wafer were used to
scan a sample simultaneously. The greatest challenge for creating multiple
probe AFM instrumentation is to get all of the probes to be as sharp as is
required for high resolution scanning.
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Calibration of Cantilever Force Constants
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As illustrated in Figure 2-40, there is considerable variability in the force
constant of an AFM cantilever because of variations primarily in the
thickness of the cantilevers. Thus, if the exact force is required for an
AFM scan or F/D curve, the force constant for the cantilever used for the
tests must be calculated. The primary method used for doing this is the Sader Method. In this method, the physical geometry of the cantilever
is measured with an optical microscope and the quality factor, Q, is
measured. These parameters are then used in an equation that calculates
the force constant, ( see Figure 2-43).
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